V5 Epitope Tag 抗体
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北京 101111
Quick Overview for V5 Epitope Tag 抗体 (ABIN962847)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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序列
- GKPIPNPLLG LDST
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特异性
- THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
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产品特性
- THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
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纯化方法
- Protein A affinity column
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免疫原
- V5 tag peptide conjugated - KLH
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亚型
- IgG2a
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anti-V5 Epitope Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 8A7A8 unconjugated
anti-V5 Epitope Tag antibody适用: 标记 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB 宿主: 兔 Polyclonal RB0413 unconjugated
anti-V5 Epitope Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-V5 Epitope Tag antibody适用: Paramyxovirus WB, ELISA, IP, IF, IHC (fro), RIA 宿主: 小鼠 Monoclonal SV5-P-K unconjugated Recombinant Antibody
anti-V5 Epitope Tag antibodyWB, ELISA, IP, DB 宿主: 小鼠 Monoclonal E10 unconjugated
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL
Western blot: 0.2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, (2014) (PubMed).
: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." in: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, (2014) (PubMed).
: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." in: Scientific reports, Vol. 4, pp. 7330, (2014) (PubMed).
: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." in: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, (2010) (PubMed).
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- V5 Epitope Tag
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别名
- V5 Tag
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物质类
- Tag
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背景
- The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
抗原
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