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Myc Tag 抗体

Name: Myc Tag 抗体
SKU: ABIN962814
Availability: OutOfStock

This anti-Myc Tag antibody is a 小鼠单克隆 antibody detecting Myc Tag in WB, IP, ELISA 和 IF. Suitable for . This Primary Antibody has been cited in 9+ publications.

Lane 1-2: Multiplex tag cell lysate (ABIN1536505) NC: 293 cell lysatePrimary antibody: Anti-c-Myc-tag Monoclonal Antibody (Mouse) (ABIN396860) The Western was performed using One-Step Western Blot Kit (ABIN491503) with 0. (Myc Tag 抗体)

Multiple Tag Cell Lysate (ABIN1536505) Primary antibody: A. (Myc Tag 抗体)

3 images

(9 references)

产品编号 ABIN962814

发货至: 中国

Datasheet as PDF

Quick Overview for Myc Tag 抗体 (ABIN962814)

抗原

Myc Tag

适用

请咨询

宿主

小鼠

克隆类型

单克隆

标记

This Myc Tag antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

克隆位点

2G8D5

品牌

THE™

特异性

THE^TM c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.

产品特性

This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

纯化方法

Protein A affinity column

免疫原

A synthetic peptide EQKLISEEDL coupled - KLH

亚型

IgG
anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag (C-Term) antibody
适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (Biotin)
适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin

anti-Myc Tag (C-Term) antibody (FITC)
适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC

anti-Myc Tag (AA 408-439) antibody
WB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated

anti-Myc Tag (AA 410-419) antibody
WB 宿主: 山羊 Polyclonal unconjugated

anti-Myc Tag (AA 408-439), (C-Term) antibody
IF, IHC, FACS, StM 宿主: 小鼠 Monoclonal 9E10-3 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05 µg/mL-0.2 µg/mL
Western blot: 0.1 µg/mL-1 µg/mL
Western blot using ONE-HOUR Western Kit: For quick results, ONE-HOUR Western Kit L00205C is recommended. 0.9 µg of this antibody is mixed with diluted WB solution (dilute 1.5 mL WB with 1.5 mL of PBST).Immunoprecipitation (IP): 1 µg/mL
Immunofluorescent staining: 1 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/mL.

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Hyun, Lee, Rim, Kumar, Han, Lee, Lee, Kim: "De-novo RNA sequencing and metabolite profiling to identify genes involved in anthocyanin biosynthesis in Korean black raspberry (Rubus coreanus Miquel)." in: PLoS ONE, Vol. 9, Issue 2, pp. e88292, (2014) (PubMed).

Ridley, Thur, Shanahan, Thillaiappan, Shen, Uhl, Walden, Rahim, Waddington, Platt, van der Spoel: "?-Glucosidase 2 (GBA2) activity and imino sugar pharmacology." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26052-66, (2013) (PubMed).

Campos, Chapman, Deymier, Bronnimann, Ozbun: "Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration." in: Journal of virology, Vol. 86, Issue 8, pp. 4169-81, (2012) (PubMed).

Zhu, Yu, Jiang, Gu: "Investigation of the influence of Arg555Trp and Thr538Pro TGFBI mutations on C-terminal cleavage and cell endoplasmic reticulum stress." in: Molecular vision, Vol. 18, pp. 1156-64, (2012) (PubMed).

Koo, Gang: "Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues." in: PLoS ONE, Vol. 7, Issue 12, pp. e51481, (2012) (PubMed).

Stringer, Piper: "A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination." in: The Journal of cell biology, Vol. 192, Issue 2, pp. 229-42, (2011) (PubMed).

Markus, Plevock, St Germain, Punch, Meaden, Lee: "Quantitative analysis of Pac1/LIS1-mediated dynein targeting: Implications for regulation of dynein activity in budding yeast." in: Cytoskeleton (Hoboken, N.J.), Vol. 68, Issue 3, pp. 157-74, (2011) (PubMed).

Faradji, Bloyer, Dardalhon-Cuménal, Randsholt, Peronnet: "Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 5, pp. 805-18, (2011) (PubMed).

Teterina, Pinto, Weaver, Jensen, Ehrenfeld: "Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells." in: Journal of virology, Vol. 85, Issue 9, pp. 4284-96, (2011) (PubMed).
抗原

Myc Tag

别名

C-Myc Tag

物质类

Tag

背景

The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.