alpha Tubulin 抗体 (C-Term, Subunit alpha)
Quick Overview for alpha Tubulin 抗体 (C-Term, Subunit alpha) (ABIN962076)
抗原
See all alpha Tubulin (TUBA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term, Subunit alpha
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品牌
- IHC-plus™
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特异性
- Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.
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纯化方法
- Affinity purified
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免疫原
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Yeast tubulin.
Type of Immunogen: Purified protein -
亚型
- IgG2a
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应用备注
- Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB
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说明
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Target Species of Antibody: Yeast
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- alpha Tubulin (TUBA1)
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别名
- Alpha Tubulin
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途径
- Microtubule Dynamics
抗原
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