CPY 抗体
(3 references)
Quick Overview for CPY 抗体 (ABIN96043)
抗原
See all CPY 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-135
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Supplier
- Rockland
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原理
- Carboxypeptidase Y Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker's Yeast].
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产品特性
- Synonyms: rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Carboxypeptidase Y [Baker's Yeast]
Immunogen Type: Native Protein
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anti-Carboxypeptidase Y (CPY) antibody
CPY 适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase Y (CPY) antibody (HRP)CPY 适用: Saccharomyces cerevisiae WB, ELISA, IHC 宿主: 兔 Polyclonal HRP
anti-Carboxypeptidase Y (CPY) antibody (Biotin)CPY 适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal Biotin
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应用备注
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Application Note: Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 90 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, (2011) (PubMed).
: "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system." in: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3998-4008, (2011) (PubMed).
: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." in: Molecular plant, (2010) (PubMed).
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: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, (2011) (PubMed).
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- CPY (Carboxypeptidase Y (CPY))
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别名
- Carboxypeptidase Y
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背景
- Background: Carboxypeptidase Y is involved in degradation of small peptides. It digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. Carboxypeptidase that catalyzes the release of a C-terminal amino acid with broad specificity. It is inhibited by ZPCK.
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基因ID
- 855343
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NCBI登录号
- NP_014026
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UniProt
- P00729
抗原
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