CA1 抗体
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北京 101111
Quick Overview for CA1 抗体 (ABIN96016)
抗原
See all CA1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-1154
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Supplier
- Rockland
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原理
- Carbonic Anhydrase I Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].
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产品特性
- Synonyms: goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Carbonic Anhydrase I [Human Erythrocytes]
Immunogen Type: Native Protein
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anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibody
CA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal M1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 2-261) antibodyCA1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal M2 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (Internal Region) antibodyCA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (N-Term) antibodyCA1 适用: 人, Cow, 豚鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 25-90) antibodyCA1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 9D6D7 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 2-261) antibodyCA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Carbonic Anhydrase I has been assayed against 1.0 μg of Carbonic Anhydrase I [Human Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 100 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CA1 (Carbonic Anhydrase I (CA1))
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别名
- CA1
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背景
- Background: Carbonic Anhydrase 1 reverses hydration of carbon dioxide and can hydrate cyanamide to urea. Carbonic Anhydrase I is activated by histamine, imidazole, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide, benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4-carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2-aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide), and 'prong inhibitors' BR15, BR17, BR22 and BR30. It is activated by a short exposition to Foscarnet (phosphonoformate trisodium salt), but inhibited by a long one. Esterase activity is weakly reduced by cyanamide.
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基因ID
- 759
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NCBI登录号
- NP_001122301
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UniProt
- P00915
抗原
-
