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CA1 抗体

CA1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN96016
Supplier Product No.: 100-1154
发货至: 中国
  • 抗原 See all CA1 抗体
    CA1 (Carbonic Anhydrase I (CA1))
    适用
    • 86
    • 33
    • 30
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 85
    • 7
    • 7
    • 1
    • 1
    山羊
    克隆类型
    • 84
    • 17
    多克隆
    标记
    • 49
    • 17
    • 7
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    This CA1 antibody is un-conjugated
    应用范围
    • 66
    • 45
    • 31
    • 15
    • 9
    • 9
    • 8
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    100-1154
    Supplier
    Rockland
    原理
    Carbonic Anhydrase I Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].
    产品特性
    Synonyms: goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Carbonic Anhydrase I [Human Erythrocytes]

    Immunogen Type: Native Protein

    Top Product
    Discover our top product CA1 Primary Antibody
  • 应用备注

    Application Note: Anti-Carbonic Anhydrase I has been assayed against 1.0 μg of Carbonic Anhydrase I [Human Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 is suggested for this product.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:20,000 - 1:100,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    100 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    CA1 (Carbonic Anhydrase I (CA1))
    别名
    CA1 (CA1 产品)
    背景
    Background: Carbonic Anhydrase 1 reverses hydration of carbon dioxide and can hydrate cyanamide to urea. Carbonic Anhydrase I is activated by histamine, imidazole, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide, benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4-carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2-aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide), and 'prong inhibitors' BR15, BR17, BR22 and BR30. It is activated by a short exposition to Foscarnet (phosphonoformate trisodium salt), but inhibited by a long one. Esterase activity is weakly reduced by cyanamide.
    基因ID
    759
    NCBI登录号
    NP_001122301
    UniProt
    P00915
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