HCV 1b NS5B 抗体 (AA 111-130)
Quick Overview for HCV 1b NS5B 抗体 (AA 111-130) (ABIN956403)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 111-130
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序列
- LSSKAVNHIR SVWKDLLEDT
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特异性
- Human, HCV subtype 1b NS5B
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产品特性
- Partially humanized, chimeric recombinant monoclonal
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纯化方法
- purifiedby protein G affinity chromatography
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免疫原
- Recombinant Hepatitis C Virus subtype 1b nonstructuralprotein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
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亚型
- IgG1
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anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 111-130) antibody
HCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 9A2 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 92-105) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 6B12 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 95-105) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 7G8 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 77-86) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 6G5 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 1-14) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) ELISA, IP 宿主: 小鼠 Monoclonal 8B2 unconjugated
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应用备注
- ELISAtiter 1:1000, Western immunoblotting, reduced conditions, dilution 1:1000, Immunofluorescense 1:1000. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml
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缓冲液
- PBS pH 7.4, with 0.1%
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Divide antibodyinto aliquots prior usage. Avoid multiple freeze-thaw cycles as productdegradation may result.
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储存条件
- -20 °C
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储存方法
- This product isshipped in non-frozen liquid form in ambient conditions
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- HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
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物质类
- Viral Protein
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背景
- Non-structural protein 5B (NS5B)represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, whichis a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronichepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunitRNA-dependent RNA polymerase is absolutely essential for the viral replication.Antibodyis derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mousehybridoma culture. cDNA of antibody variable regions were isolated and clonedinto the human IgG1 constant region-containing antibody expression vector.Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cellline. Antibody is used for high-specific efficient detection of soluble ormembrane-bound proteins fused with 9A2 epitope-tag sequence. For moreinformation please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview
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UniProt
- Q9WMX2
抗原
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