Biotin 抗体 (HRP)
Quick Overview for Biotin 抗体 (HRP) (ABIN95620)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-103-098
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Supplier
- Rockland
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原理
- Biotin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.
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产品特性
- Synonyms: goat anti-Biotin Antibody HRP Conjugation, Peroxidase conjugated goat anti-Biotin Antibody
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
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免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other
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亚型
- IgG
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anti-Biotin antibody
WB, ELISA, IHC, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Biotin Peroxidase conjugated antibody has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:3,000 - 1:15,000
ELISA Dilution: 1:140,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Peroxidase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
抗原
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