Avidin 抗体
Quick Overview for Avidin 抗体 (ABIN95469)
抗原
See all Avidin (AVD) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4197-0100
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Supplier
- Rockland
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交叉反应 （详细）
- No cross reactivity occurs against Streptavidin.
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纯度
- Anti-AVIDIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
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内毒素水平
- Low Endotoxin : No
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免疫原
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Avidin (Hen Egg White)
Immunogen Type: Native Protein -
亚型
- IgG
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anti-Avidin (AVD) antibody (Cy3)
AVD 适用: Chicken egg IHC (fro), IHC (p), ICC 宿主: Chicken eggs Polyclonal Cy3
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, DB, IP 宿主: 兔 Polyclonal unconjugated
anti-Avidin (AVD) antibody (Biotin)AVD 适用: Chicken egg WB, ELISA, IHC, IM 宿主: 兔 Polyclonal Biotin
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应用备注
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Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2.000 - 1:20.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:1.000 - 1:10.000 -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
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有效期
- Expiration date is one (1) year from date of opening.
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- Avidin (AVD)
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别名
- AVIDIN
抗原
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