Aspartate Transaminase 抗体
Quick Overview for Aspartate Transaminase 抗体 (ABIN95406)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-601-144-0100
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Supplier
- Rockland
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原理
- Aspartate Transaminase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart].
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产品特性
- Synonyms: sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody, AST
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纯化方法
- Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Aspartate Transaminase (Ast) antibody (HRP)
Ast 适用: Pig ELISA, WB, DB 宿主: 绵羊 Polyclonal HRP
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应用备注
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Application Note: Anti-Aspartate Transaminase is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Aspartate Transaminase (Ast)
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别名
- Aspartate Transaminase
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背景
- Background: Anti-Aspartate Transaminase is detects Aspartate Transaminase. Aspartate Transaminase is a pyridoxal phosphate (PLP)-dependent transaminase enzyme. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. Anti-Aspartate Transaminase antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.
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基因ID
- 396967
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NCBI登录号
- NP_999092
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UniProt
- P00503
抗原
-
