Aspartate Transaminase 抗体
(1 reference)
Quick Overview for Aspartate Transaminase 抗体 (ABIN95405)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-601-144
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Supplier
- Rockland
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原理
- Aspartate Transaminase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
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产品特性
- Synonyms: sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein
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anti-Aspartate Transaminase (Ast) antibody (HRP)
Ast 适用: Pig ELISA, WB, DB 宿主: 绵羊 Polyclonal HRP
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应用备注
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Application Note: Anti-Aspartate Transaminase has been assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 96 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, (2011) (PubMed).
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: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, (2011) (PubMed).
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- Aspartate Transaminase (Ast)
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别名
- Aspartate Transaminase
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背景
- Background: Aspartate aminotransferase is involved in the biosynthesis of L-glutamate from L-aspartate or L-cysteine. It is an important regulator of the levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, Aspartate aminotransferase regulates levels of mercaptopyruvate, an important source of hydrogen sulfide, then mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In eukaryotes there are cytoplasmic, mitochondrial, and chloroplastic isozymes.
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基因ID
- 396967
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NCBI登录号
- NP_999092
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UniProt
- P00503
抗原
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