AnsB 抗体
Quick Overview for AnsB 抗体 (ABIN95394)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-4171
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Supplier
- Rockland
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原理
- L-Asparaginase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Asparaginase [Escherichia coli].
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产品特性
- Synonyms: rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Asparaginase [Escherichia coli]
Immunogen Type: Native Protein
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anti-AnsB antibody (Biotin)
适用: E. coli ELISA, WB, DB 宿主: 兔 Polyclonal Biotin
anti-AnsB antibody (HRP)适用: E. coli ELISA, WB 宿主: 兔 Polyclonal HRP
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-L-asparaginase has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 65 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- AnsB
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背景
- Background: L-asparaginase from E.coli has two isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
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基因ID
- 1039460
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UniProt
- P00805
抗原
-
