Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
10 mg IgY/ml (based on absorbance at 280 nm)
缓冲液
10 mM PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted in the dark at 2-8 °C.
抗原
ACPP
(Acid Phosphatase, Prostate (ACPP))
别名
ACPP / Prostatic Acid Phosphatase
背景
Mouse PAP is a 43,698 dalton protein (381 amino acids, NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase