Amylase, alpha 抗体
Quick Overview for Amylase, alpha 抗体 (ABIN95004)
抗原
See all Amylase, alpha (AMY) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-401-045
-
Supplier
- Rockland
-
原理
- Alpha Amylase Antibody
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].
-
产品特性
- Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase
-
纯化方法
- Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Amylase, alpha (AMY) antibody
AMY 适用: Oryza sativa WB 宿主: 兔 Polyclonal unconjugated
anti-Amylase, alpha (AMY) antibody (Biotin)AMY 适用: Bacillus amyloliquefaciens WB, ELISA, IP 宿主: 兔 Polyclonal Biotin
-
-
应用备注
-
Application Note: Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 μg of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store Alpha Amylase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Alpha Amylase Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Amylase, alpha (AMY)
-
别名
- Alpha-amylase
-
背景
- Background: Alpha Amylase is a protein enzyme that preforms endohydrolysis of alpha bonds in large alpha linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Alpha Amylase applications include ethanol production and reduction of starch or glycogen molecules. Anti-Alpha Amylase Antibody is useful for researchers interested in Metabolism, Cancer, Cell Signaling, and Signal Transduction research.
-
基因ID
- 7849308
-
NCBI登录号
- WP_013352208
-
UniProt
- P00692
抗原
-
