Amylase, alpha 抗体
(1 reference)
Quick Overview for Amylase, alpha 抗体 (ABIN95003)
抗原
See all Amylase, alpha (AMY) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-4145
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Supplier
- Rockland
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原理
- Alpha Amylase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified a-Amylase [Bacillus amyloliquefaciens].
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产品特性
- Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase
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纯化方法
- Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein
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anti-Amylase, alpha (AMY) antibody
AMY 适用: Oryza sativa WB 宿主: 兔 Polyclonal unconjugated
anti-Amylase, alpha (AMY) antibody (Biotin)AMY 适用: Bacillus amyloliquefaciens WB, ELISA, IP 宿主: 兔 Polyclonal Biotin
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应用备注
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Application Note: Anti-Alpha Amylase is useful in ELISA, WB, and IP. User Optimization is suggested.
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 85 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Properties and applications of starch-converting enzymes of the alpha-amylase family." in: Journal of biotechnology, Vol. 94, Issue 2, pp. 137-55, (2002) (PubMed).
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: "Properties and applications of starch-converting enzymes of the alpha-amylase family." in: Journal of biotechnology, Vol. 94, Issue 2, pp. 137-55, (2002) (PubMed).
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- Amylase, alpha (AMY)
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别名
- Alpha-amylase
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背景
- Background: Alpha Amylase is an enzyme that begins the digestion of starches. Specifically, Alpha Amylase cleaves the alpha bonds in large polysaccharides. Alpha Amylase is found in both the pancreas and saliva in humans, with salivary amylase beginning the digestion of starches and pancreatic amylase finishing the digestion. The large amount of conserved amino acid sequences and prevalence of alpha amylase enzymes has allowed this class of enzymes to be beneficial to industrial breakdown of starches into glucose and high-fructose corn syrup. Alpha amylase derived from bacillus amyloliquefaciens is also useful in the production of various detergents relying on the breakdown of starches. Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody is ideal for investigators in Enzymology, Molecular Biology, and Microbiology research.
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基因ID
- 7849308
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NCBI登录号
- WP_013352208
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UniProt
- P00692
抗原
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