D Amino Acid Oxidase 抗体
Quick Overview for D Amino Acid Oxidase 抗体 (ABIN94968)
抗原
See all D Amino Acid Oxidase (DAO) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-601-143-0100
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Supplier
- Rockland
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原理
- D-Amino Acid Oxidase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].
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产品特性
- Synonyms: sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO
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纯化方法
- Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: D-Amino Acid Oxidase [Pig Kidney]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-D-Amino-Acid Oxidase (DAO) (AA 1-347) antibody
DAO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (C-Term) antibodyDAO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 1-347) antibodyDAO 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 1-347) antibodyDAO 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) antibodyDAO 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 286-298) antibodyVerified DAO 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 216-248) antibodyDAO 适用: 人 WB 宿主: 兔 Polyclonal RB52983 unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 119-218) antibodyDAO 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (C-Term) antibodyVerified DAO 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-D-Amino-Acid Oxidase (DAO) (AA 119-218) antibodyDAO 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F5 unconjugated
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应用备注
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Application Note: D-AMINO ACID OXIDASE antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
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别名
- DAO
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背景
- Background: D-Amino Acid Oxidase regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. It acts on a variety of D-amino acids, with a preference for those having small hydrophobic side chains, followed by those bearing polar, aromatic, and basic groups. D-Amino Acid Oxidase does not act on acidic amino acids.
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基因ID
- 397134
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NCBI登录号
- NP_999231
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UniProt
- P00371
抗原
-
