ALDOA 抗体
(1 reference)
Quick Overview for ALDOA 抗体 (ABIN94900)
抗原
See all ALDOA 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-1141
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Supplier
- Rockland
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原理
- Aldolase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Aldolase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Aldolase [Rabbit Muscle]
Immunogen Type: Native Protein
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anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 18-273) antibody
ALDOA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 1-364) antibodyALDOA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3D9-6F3 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 9-145) antibodyALDOA 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1C5B2 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 1-364) antibodyALDOA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C12-6D11 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 9-145) antibodyALDOA 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2H2B1 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 303-331) antibodyALDOA 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 66-95) antibodyALDOA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 7D8 unconjugated Recombinant Antibody
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人, 兔 WB, ELISA, IHC, IF, IP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Aldolase has been tested by immunoprecipitation and western blot and is suitable to be assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 µL of antibody to immunoprecipitate 50 µL of protein lysate.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 90 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Involvement of splicing factor-1 in beta-catenin/T-cell factor-4-mediated gene transactivation and pre-mRNA splicing." in: Gastroenterology, Vol. 132, Issue 3, pp. 1039-54, (2007) (PubMed).
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: "Involvement of splicing factor-1 in beta-catenin/T-cell factor-4-mediated gene transactivation and pre-mRNA splicing." in: Gastroenterology, Vol. 132, Issue 3, pp. 1039-54, (2007) (PubMed).
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- ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))
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别名
- ALDOA
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背景
- Background: Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
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基因ID
- 100009055
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NCBI登录号
- NP_001075707
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UniProt
- P00883
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途径
- Ribonucleoside Biosynthetic Process
抗原
-
