ALT 抗体
Quick Overview for ALT 抗体 (ABIN94798)
抗原
See all ALT 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-601-206
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Supplier
- Rockland
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原理
- Alanine Transaminase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alanine Transaminase (ALT) [Pig Heart].
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产品特性
- Synonyms: sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Alanine Transaminase (ALT) [Pig Heart]
Immunogen Type: Native Protein
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anti-Alanine Aminotransferase (ALT) (AA 367-480) antibody
ALT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 147-263) antibodyALT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 143-417) antibodyALT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 581-738) antibodyALT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 2-496) antibodyALT 适用: 人 WB, IHC, IP, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 255-492) antibodyALT 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Alanine Aminotransferase (ALT) (AA 255-492) antibodyALT 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 1-496) antibodyALT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E2 unconjugated
anti-Alanine Aminotransferase (ALT) antibodyALT 适用: 人, 大鼠, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alanine Aminotransferase (ALT) (AA 720-961) antibodyALT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Alanine Transaminase has been assayed against 1.0 μg of Alanine Transaminase (ALT) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 96 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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别名
- GPT
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背景
- Background: Alanine Transaminase catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
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基因ID
- 2875, 113565
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UniProt
- P13191
抗原
-
