Acetylcholinesterase 抗体 (Biotin)
Quick Overview for Acetylcholinesterase 抗体 (Biotin) (ABIN94540)
抗原
See all Acetylcholinesterase (AChE) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-106-232-0100
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Supplier
- Rockland
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原理
- Acetyl Cholinesterase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].
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产品特性
- Synonyms: goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE
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纯化方法
- Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Acetylcholinesterase (AChE) (C-Term) antibody
Verified AChE 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 398-617) antibodyAChE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (C-Term) antibodyAChE 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 157-332) antibodyAChE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175), (N-Term) antibodyAChE 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB16792 unconjugated
anti-Acetylcholinesterase (AChE) (AA 521-614) antibodyAChE 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 53-162) antibodyAChE 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) antibodyAChE 适用: 人 ELISA, IHC, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 366-554) antibodyAChE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175) antibodyAChE 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Acetylcholinesterase (AChE)
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别名
- ACHE
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背景
- Background: Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research.
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基因ID
- 540446
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NCBI登录号
- NP_001069688
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UniProt
- P23795
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途径
- Skeletal Muscle Fiber Development
抗原
-
