Acrosin 抗体
(7 references)Quick Overview for Acrosin 抗体 (ABIN94455)
抗原
See all Acrosin (ACR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Por Acrosin Purified
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特异性
- The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
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无交叉反应
- Cow, 犬, 人
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交叉反应 （详细）
- Porcine
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
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亚型
- IgG1
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anti-Acrosin (ACR) (N-Term) antibody
ACR 适用: 人 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (AA 201-300) antibodyACR 适用: 人, 大鼠, 小鼠, 山羊 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (AA 43-291) antibodyACR 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (Internal Region) antibodyVerified ACR 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Acrosin (ACR) (AA 99-127) antibodyACR 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB16796 unconjugated
anti-Acrosin (ACR) (AA 106-135), (Middle Region) antibodyACR 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (N-Term) antibodyACR 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (AA 231-280) antibodyACR 适用: 人, Cow, 马, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosin (ACR) (AA 387-416), (C-Term) antibodyACR 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB16798 unconjugated
anti-Acrosin (ACR) (AA 99-127) antibodyACR 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
Immunocytochemistry: Membrane permeabilization (acetone) is essential. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Fluorescent analysis of boar sperm capacitation process in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).
: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).
: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).
: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).
: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).
: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).
: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).
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: "Fluorescent analysis of boar sperm capacitation process in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).
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- Acrosin (ACR)
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别名
- Acrosin
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背景
- Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53
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基因ID
- 397098
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UniProt
- P08001
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途径
- cAMP Metabolic Process
抗原
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