NEFM 抗体
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北京 101111
Quick Overview for NEFM 抗体 (ABIN94435)
抗原
See all NEFM 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Neurofilament M Purified
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特异性
- The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa intracellular antigen) of various species.
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交叉反应 （详细）
- Mammalian
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
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亚型
- IgG2a
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anti-Neurofilament Triplet M Protein (NEFM) (AA 779-916) antibody
NEFM 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B6B12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 381-443) antibodyNEFM 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 101-200) antibodyNEFM 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 105-287) antibodyNEFM 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 200-300) antibodyNEFM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyKD Validated NEFM 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB5745 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 3H11 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (Internal Region) antibodyVerified NEFM 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyKD Validated NEFM 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB5825 unconjugated
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应用备注
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Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1 % glycine + 0.2 % gelatin 10 min.
Western blotting: Recommended dilution: 1-2 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." in: Folia biologica, Vol. 45, Issue 4, pp. 163-5, (2000) (PubMed).
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: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." in: Folia biologica, Vol. 45, Issue 4, pp. 163-5, (2000) (PubMed).
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- NEFM (Neurofilament Triplet M Protein (NEFM))
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别名
- Neurofilament M
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背景
- Neurofilament medium,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFM, NEFM, Neurofilament medium chain, NEF3
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基因ID
- 4741
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UniProt
- P07197
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途径
- Brown Fat Cell Differentiation
抗原
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