Kinesin (heavy chain) 抗体
适用: Pig, 人, 小鼠, 大鼠
ICC
宿主: 小鼠
Monoclonal
KN-03
unconjugated
(1 reference)-
- 抗原
- Kinesin (heavy chain)
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适用
- Pig, 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunocytochemistry (ICC)
- 特异性
- The antibody KN-03 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.
- 交叉反应 （详细）
- Mouse, Human, Rat, Other not tested, Porcine
- 纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Enriched fraction of porcine brain kinesin.
- 克隆位点
- KN-03
- 亚型
- IgM
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anti-Kinesin (heavy chain) antibody
适用: Pig, 人, 小鼠, 大鼠 ICC 宿主: 小鼠 Monoclonal KN-02 unconjugated
anti-Kinesin (heavy chain) antibody适用: Pig ICC, IF 宿主: 小鼠 Monoclonal KN-02 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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-
: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).
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: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).
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- 抗原
- Kinesin (heavy chain)
- 别名
- KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, kinesin family member 5B antibody, kinesin family member 5C antibody, KIF5B antibody, Kif5c antibody
- 背景
- Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KHC, KINHC, KIF5
- 途径
- Peptide Hormone Metabolism
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