Ig Light Chains 抗体
Cited in 1 publication. This 小鼠 单克隆 anti-not_set antibody (Clone IVA285-1) specifically detects not_set in FACS, IHC (fro) 和 WB. The antibody is reactive with Cow 和 绵羊 samples.
(1 reference)
产品编号 ABIN94401
发货至:
中国
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Quick Overview for Ig Light Chains 抗体 (ABIN94401)
抗原
Ig Light Chains
适用
Cow, 绵羊
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
克隆位点
IVA285-1
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原理
- Anti-Bov Ig Light Chains Purified
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特异性
- The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
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交叉反应 （详细）
- Bovine, Sheep
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Fraction of Bovine immunoglobulin.
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亚型
- IgG1
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anti-Ig Light Chains antibody
适用: 人 FACS, ELISA, ICC, IHC 宿主: 兔 Monoclonal RM129 unconjugated Recombinant Antibody
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应用备注
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Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
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: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
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- Ig Light Chains
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背景
- Immunoglobulin light chains
抗原
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