GAPDHS 抗体
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北京 101111
Quick Overview for GAPDHS 抗体 (ABIN94395)
抗原
See all GAPDHS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-GAPDHS Purified
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特异性
- The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
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交叉反应 （详细）
- Human, Porcine
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纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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亚型
- IgM
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibody
GAPDHS 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E3-2E10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: 人 WB, IHC (p), IHC 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 104-134) antibodyGAPDHS 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB16542 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 251-350) antibodyGAPDHS 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 107-156) antibodyGAPDHS 适用: 人, 小鼠, 猴, Pig, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibody (FITC)GAPDHS 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal Hs-8 FITC
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (N-Term) antibodyGAPDHS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 μg/mL. Intraacrosomal staining. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))
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别名
- GAPDHS
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背景
- Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35
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基因ID
- 26330
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UniProt
- O14556
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
-
