FYN 抗体 (AA 7-176)
(2 references)Quick Overview for FYN 抗体 (AA 7-176) (ABIN94311)
抗原
See all FYN 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 7-176
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原理
- Anti-FYN Purified
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特异性
- The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
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交叉反应 （详细）
- Mouse, Human, Other not tested
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
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亚型
- IgG2b
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anti-FYN Oncogene Related To SRC, FGR, YES (FYN) antibody
FYN 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2A10 unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr530) antibodyFYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 13-43) antibodyFYN 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-537) antibodyFYN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-30) antibodyFYN 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr530) antibodyFYN 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr530) antibodyFYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-482) antibodyFYN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3G11-F9 unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-90) antibodyFYN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A8 unconjugated
anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (C-Term) antibodyFYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: brain.
Western blotting: Recommended dilution: 2 μg/mL, positive control: JURKAT human T cell leukemia cell line.
Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, (2011) (PubMed).
: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).
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: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, (2011) (PubMed).
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- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
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别名
- FYN
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背景
- FYN proto-oncogene, Src family tyrosine kinase,Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.,p59Fyn, SLK, SYN
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基因ID
- 2534
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UniProt
- P06241
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途径
- JAK/STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
抗原
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