CD45RB 抗体
(1 reference)-
- 抗原 See all CD45RB 抗体
- CD45RB
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45RB antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- The antibody MEM-143 reacts with an extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Human peripheral blood lymphocytes.
- 克隆位点
- MEM-143
- 亚型
- IgG1
- Top Product
- Discover our top product CD45RB Primary Antibody
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anti-CD45RB (CD45RB) antibody
CD45RB 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-55 unconjugated
anti-CD45RB (CD45RB) antibody (FITC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 FITC
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 APC
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 PE
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A PE
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 人, 猴, 猕猴, 兔 FACS, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-55 unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 人, 猴, 猕猴, 兔 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM55 unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A unconjugated
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, 猴, 猕猴 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-55 APC
anti-CD45RB (CD45RB) antibody (Biotin)CD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A Biotin
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- 应用备注
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Flow cytometry: Recommended dilution: 0.5-4 μg/mL. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: human tonsil. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).
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: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).
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- 抗原
- CD45RB
- 别名
- CD45RB (CD45RB 产品)
- 背景
- CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.,PTPRCR, T200RB
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