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TERT 抗体 (AA 597-611)

This anti-TERT antibody is a 山羊 多克隆 antibody detecting TERT in ELISA, FACS 和 IF. Suitable for 人.
产品编号 ABIN940568
发货至: 中国

Quick Overview for TERT 抗体 (AA 597-611) (ABIN940568)

抗原

See all TERT 抗体
TERT (Telomerase Reverse Transcriptase (TERT))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This TERT antibody is un-conjugated

应用范围

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ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    AA 597-611

    原理

    TERT (aa597-611)

    序列

    QLRELSEAEV RQHRE

    特异性

    This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    TERT (Telomerase Reverse Transcriptase (TERT))

    别名

    TERT

    背景

    TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2

    基因ID

    7015

    NCBI登录号

    NP_937983, NP_001180305

    途径

    Telomere Maintenance
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