CD147 抗体 (FITC)
(2 references)Quick Overview for CD147 抗体 (FITC) (ABIN93983)
抗原
See all CD147 (BSG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD147 FITC
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特异性
- The antibody MEM-M6/1 recognizes an extracellular epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
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交叉反应 （详细）
- Human
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纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
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亚型
- IgG1
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anti-Basigin (Ok Blood Group) (BSG) (AA 79-280) antibody
BSG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 87-323) antibodyBSG 适用: 小鼠 WB, FACS, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 59-321) antibodyBSG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 30-200) antibodyBSG 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7H4D4 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 2D8C11 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 WB, FACS, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3E10C1 unconjugated
anti-Basigin (Ok Blood Group) (BSG) antibodyBSG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal BSG-963 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 1-269) antibodyBSG 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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溶解方式
- No reconstitution is necessary.
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
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- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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别名
- CD147
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背景
- Basigin (Ok blood group),CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.,Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group
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基因ID
- 682
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UniProt
- P35613
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途径
- S100 Proteins
抗原
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