ACRV1 抗体
Quick Overview for ACRV1 抗体 (ABIN93928)
抗原
See all ACRV1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Can Intra-Acrosomal Protein Purified
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特异性
- The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
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交叉反应 (详细)
- Canine (Dog)
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
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亚型
- IgG
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应用备注
- Immunocytochemistry: Membrane permeabilization (acetone) is essential.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).
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: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).
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- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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别名
- Intra-Acrosomal Protein
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背景
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can
抗原
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