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ACRV1 抗体

Cited in 1 publication. This 小鼠 单克隆 anti-ACRV1 antibody (Clone Ds-2) specifically detects ACRV1 in WB 和 ICC. The antibody is reactive with 犬 samples.
产品编号 ABIN93928
发货至: 中国
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Quick Overview for ACRV1 抗体 (ABIN93928)

抗原

See all ACRV1 抗体
ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This ACRV1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC)

克隆位点

Ds-2
  • 原理

    Anti-Can Intra-Acrosomal Protein Purified

    特异性

    The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.

    交叉反应 (详细)

    Canine (Dog)

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.

    亚型

    IgG
  • 应用备注

    Immunocytochemistry: Membrane permeabilization (acetone) is essential.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Geussová, P?knicová, Capková, Kaláb, Moos, Philimonenko, Hozák: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).

  • 抗原

    ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

    别名

    Intra-Acrosomal Protein

    背景

    One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can
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