Blood Group Lewis B 抗体
This 小鼠 单克隆 antibody specifically detects Blood Group Lewis B in IHC (p), WB 和 ELISA. It exhibits reactivity toward 人. It has been mentioned in 2+ publications
(2 references)
产品编号 ABIN93925
发货至:
中国
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Quick Overview for Blood Group Lewis B 抗体 (ABIN93925)
抗原
Blood Group Lewis B
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Blood Group Lewis B antibody is un-conjugated
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
克隆位点
2-25LE
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原理
- Anti-Blood Group Lewis b Purified
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特异性
- The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.
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交叉反应 （详细）
- Other not tested, Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
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亚型
- IgG1
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anti-Blood Group Lewis B antibody
适用: 人 IHC (p), WB, EIA 宿主: 小鼠 Monoclonal 2-25LE unconjugated
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应用备注
- Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation: 1 hour / RT, detection DAB.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
"First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." in: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).
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: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
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- Blood Group Lewis B
抗原
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