Blood Group Lewis A 抗体
适用: 人
IHC (p), WB, ELISA
宿主: 小鼠
Monoclonal
7LE
unconjugated
(2 references)-
- 抗原
- Blood Group Lewis A
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
- 特异性
- The mouse monoclonal antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
- 交叉反应 （详细）
- Other not tested, Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
- 克隆位点
- 7LE
- 亚型
- IgG1
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anti-Blood Group Lewis A antibody
适用: 人, 小鼠 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal 7LE unconjugated
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- 应用备注
- Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour / RT, detection DAB.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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-
: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
"First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." in: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).
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: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
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- 抗原
- Blood Group Lewis A
- 别名
- CD174 antibody, FT3B antibody, FucT-III antibody, LE antibody, Les antibody, fucosyltransferase 3 (Lewis blood group) antibody, FUT3 antibody
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