Blood Group Lewis A 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in WB, IHC (p) 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
(2 references)Quick Overview for Blood Group Lewis A 抗体 (ABIN93924)
抗原
Blood Group Lewis A
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
克隆位点
7LE
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原理
- Anti-Blood Group Lewis a Purified
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特异性
- The mouse monoclonal antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
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交叉反应 （详细）
- Other not tested, Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
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亚型
- IgG1
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anti-Blood Group Lewis A antibody
适用: 人, 小鼠 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal 7LE unconjugated
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应用备注
- Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour / RT, detection DAB.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
"First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." in: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).
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: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).
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- Blood Group Lewis A
抗原
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