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TUBB 抗体

TUBB 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ICC 宿主: 小鼠 Monoclonal TU-12 unconjugated
产品编号 ABIN93915
发货至: 中国
  • 抗原 See all TUBB 抗体
    TUBB (Tubulin, beta (TUBB))
    适用
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    人, 小鼠, 大鼠, Pig
    宿主
    • 110
    • 75
    • 1
    • 1
    • 1
    小鼠
    克隆类型
    • 96
    • 88
    • 2
    单克隆
    标记
    • 99
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    This TUBB antibody is un-conjugated
    应用范围
    • 167
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    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    特异性
    The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
    交叉反应 (详细)
    Broad species reactivity
    纯化方法
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    Porcine brain microtubule protein MTP-1.
    克隆位点
    TU-12
    亚型
    IgM
    Top Product
    Discover our top product TUBB Primary Antibody
  • 应用备注
    Immunocytochemistry: Recommended dilution: 2-5 μg/mL, fixed and permeabilized cells.
    Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

  • 抗原
    TUBB (Tubulin, beta (TUBB))
    别名
    beta-tubulin (TUBB 产品)
    别名
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    背景
    Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB
    基因ID
    81027
    UniProt
    Q9H4B7
    途径
    Microtubule Dynamics, M Phase
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