TUBB 抗体
(1 reference)Quick Overview for TUBB 抗体 (ABIN93915)
抗原
See all TUBB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-beta-Tubulin Purified
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特异性
- The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
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交叉反应 （详细）
- Broad species reactivity
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纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Porcine brain microtubule protein MTP-1.
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亚型
- IgM
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anti-Tubulin, beta (TUBB) antibody
TUBB 适用: 人, 小鼠, 大鼠, Pig, 小鸡, Tetrahymena, 鱼, Nicotiana tabacum, Arabidopsis, Paramecium WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal TU-06 unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人 WB, IF, ICC, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 61-160) antibodyTUBB 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Tubulin, beta (TUBB) (AA 1-444) antibodyTUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B3 unconjugated
anti-Tubulin, beta (TUBB) (AA 160-447) antibodyTUBB 适用: 人, 小鼠 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 87CT59-3-7 unconjugated
anti-Tubulin, beta (TUBB) (AA 1-50) antibodyTUBB 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry: Recommended dilution: 2-5 μg/mL, fixed and permeabilized cells.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).
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: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).
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- TUBB (Tubulin, beta (TUBB))
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别名
- beta-Tubulin
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背景
- Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB
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基因ID
- 81027
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UniProt
- Q9H4B7
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途径
- Microtubule Dynamics, M Phase
抗原
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