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TUBB3 抗体 (N-Term) (FITC)

This anti-TUBB3 antibody is a 小鼠 单克隆 antibody detecting TUBB3 in FACS 和 ICC. Suitable for 人, 小鼠, 大鼠, 犬 和 Pig. This Primary Antibody has been cited in 10+ publications.
产品编号 ABIN93912
发货至: 中国

Quick Overview for TUBB3 抗体 (N-Term) (FITC) (ABIN93912)

抗原

See all TUBB3 抗体
TUBB3 (Tubulin, beta 3 (TUBB3))

适用

  • 157
  • 75
  • 68
  • 20
  • 9
  • 7
  • 7
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
人, 小鼠, 大鼠, 犬, Pig

宿主

  • 92
  • 73
  • 4
  • 1
  • 1
小鼠

克隆类型

  • 93
  • 78
单克隆

标记

  • 93
  • 13
  • 10
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This TUBB3 antibody is conjugated to FITC

应用范围

  • 140
  • 76
  • 39
  • 39
  • 31
  • 27
  • 25
  • 22
  • 21
  • 15
  • 14
  • 14
  • 6
  • 5
  • 4
  • 4
  • 1
Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

TU-20
  • 抗原表位

    • 28
    • 18
    • 16
    • 14
    • 11
    • 9
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    原理

    Anti-betaIII-Tubulin FITC

    特异性

    The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

    交叉反应 (详细)

    Broad species reactivity

    纯化方法

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    免疫原

    Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

    亚型

    IgG1
  • 应用备注

    Flow cytometry: Recommended dilution: 1-3 μg/mL. Intracellular staining.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.
    Avoid prolonged exposure to light.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

    Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).

    Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

    Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

    Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

    Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).

    Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

    P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).

    Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).

    Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).

  • 抗原

    TUBB3 (Tubulin, beta 3 (TUBB3))

    别名

    betaIII-Tubulin

    背景

    Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3

    基因ID

    10381

    UniProt

    Q13509

    途径

    Microtubule Dynamics, M Phase
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