TUBB3 抗体 (N-Term) (FITC)
(10 references)Quick Overview for TUBB3 抗体 (N-Term) (FITC) (ABIN93912)
抗原
See all TUBB3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Anti-betaIII-Tubulin FITC
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特异性
- The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
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交叉反应 （详细）
- Broad species reactivity
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纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
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亚型
- IgG1
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anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody
TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, IHC (p), ICC, ICFC 宿主: 小鼠 Monoclonal TU-20 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibodyTUBB3 适用: 人, 小鼠, 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 8E3A7 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21098 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠, 大鼠 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 401-450) antibodyTUBB3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 1-3 μg/mL. Intracellular staining.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).
: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).
: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).
: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).
: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).
: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).
: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).
: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).
: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).
: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).
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: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).
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- TUBB3 (Tubulin, beta 3 (TUBB3))
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别名
- betaIII-Tubulin
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背景
- Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3
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基因ID
- 10381
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UniProt
- Q13509
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途径
- Microtubule Dynamics, M Phase
抗原
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