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TUBB3 抗体 (N-Term)

Name: TUBB3 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN93911
Availability: OutOfStock

TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, IHC (p), ICC, ICFC 宿主: 小鼠 Monoclonal TU-20 unconjugated

Western blotting analysis of human betaIII-tubulin using mouse monoclonal antibody TU-20 on lysates of U87 cells and Neuro 2a cells (and Jurkat cells as a negative control) under reducing and non-reducing conditions. (TUBB3 抗体 (N-Term))

Immunohistochemistry staining of betaIII tubulin (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). (TUBB3 抗体 (N-Term))

IHC (p)

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. (TUBB3 抗体 (N-Term))

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml). (TUBB3 抗体 (N-Term))

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. (TUBB3 抗体 (N-Term))

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20, green, 3 μg/mL). (TUBB3 抗体 (N-Term))

9 images

(15 references)

产品编号 ABIN93911

发货至: 中国

Datasheet as PDF

抗原 See all TUBB3 抗体

TUBB3 (Tubulin, beta 3 (TUBB3))
抗原表位
15

15

13

11

7

6

4

4

3

2

1

1

1

1

1

1

1

1

1

1
N-Term
适用
117

67

59

17

7

4

4

3

3

3

2

2

2

2

1

1
人, 小鼠, 大鼠, 犬, Pig
宿主
78

44

4

1

1
小鼠
Compatible Secondaries
克隆类型
68

60
单克隆
标记
74

12

5

3

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
This TUBB3 antibody is un-conjugated
应用范围
97

40

36

31

26

21

19

15

14

13

13

6

4

4

4
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

原理

Anti-betaIII-Tubulin Purified

特异性

The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

交叉反应（详细）

Broad species reactivity

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

克隆位点

TU-20

亚型

IgG1

Top Product

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anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody
TUBB3 适用: 人, 小鼠, 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 8E3A7 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibody
TUBB3 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21098 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibody
TUBB3 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人, 小鼠, 大鼠 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 401-450) antibody
TUBB3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) antibody
TUBB3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 小鼠 Monoclonal unconjugated

anti-Tubulin, beta 3 (TUBB3) (AA 1-210) antibody
TUBB3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: Recommended dilution: 1-2 μg/mL, positive control: porcine brain lysate, negative control: HPB-ALL human peripheral blood leukemia cell line, reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, standard ABC technique (DAB+), pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or high temperature citrate buffer antigen retrieval, positive tissue: neuronal tissue.
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Letellier, Szíber, Chamma, Saphy, Papasideri, Tessier, Sainlos, Czöndör, Thoumine: "A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation." in: Nature communications, Vol. 9, Issue 1, pp. 3979, (2019) (PubMed).

Gudernova, Balek, Varecha, Kucerova, Kunova Bosakova, Fafilek, Palusova, Uldrijan, Trantirek, Krejci: "Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480." in: Oncotarget, Vol. 8, Issue 65, pp. 109319-109331, (2017) (PubMed).

Szíber, Liliom, Morales, Ignácz, Rátkai, Ellwanger, Link, Szűcs, Hausser, Schlett: "Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover." in: Molecular biology of the cell, Vol. 28, Issue 2, pp. 285-295, (2017) (PubMed).

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, (2011) (PubMed).

Kunova, Matulka, Eiselleova, Trckova, Hampl, Dvorak: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, (2010) (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).
抗原

TUBB3 (Tubulin, beta 3 (TUBB3))

别名

betaIII-Tubulin (TUBB3 产品)

背景

Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3

基因ID

10381

UniProt

Q13509

途径

Microtubule Dynamics, M Phase