TUBB3 抗体 (N-Term)
(15 references)Quick Overview for TUBB3 抗体 (N-Term) (ABIN93911)
抗原
See all TUBB3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Anti-betaIII-Tubulin Purified
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特异性
- The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
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交叉反应 （详细）
- Broad species reactivity
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
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亚型
- IgG1
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anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody (FITC)
TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig FACS, ICC 宿主: 小鼠 Monoclonal TU-20 FITC
anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibodyTUBB3 适用: 人, 小鼠, 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 8E3A7 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21098 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠, 大鼠 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 401-450) antibodyTUBB3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 1-2 μg/mL, positive control: porcine brain lysate, negative control: HPB-ALL human peripheral blood leukemia cell line, reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, standard ABC technique (DAB+), pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or high temperature citrate buffer antigen retrieval, positive tissue: neuronal tissue.
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation." in: Nature communications, Vol. 9, Issue 1, pp. 3979, (2019) (PubMed).
: "Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480." in: Oncotarget, Vol. 8, Issue 65, pp. 109319-109331, (2017) (PubMed).
: "Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover." in: Molecular biology of the cell, Vol. 28, Issue 2, pp. 285-295, (2017) (PubMed).
: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).
: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, (2011) (PubMed).
: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, (2010) (PubMed).
: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).
: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, (2009) (PubMed).
: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).
: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).
: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, (2007) (PubMed).
: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).
: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, (2001) (PubMed).
: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, (2000) (PubMed).
: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, (1998) (PubMed).
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: "A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation." in: Nature communications, Vol. 9, Issue 1, pp. 3979, (2019) (PubMed).
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- TUBB3 (Tubulin, beta 3 (TUBB3))
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别名
- betaIII-Tubulin
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背景
- Tubulin beta 3,The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.,TUBB3
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基因ID
- 10381
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UniProt
- Q13509
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途径
- Microtubule Dynamics, M Phase
抗原
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