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beta-2 Microglobulin 抗体 (FITC)

Name: beta-2 Microglobulin 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN93905
Availability: OutOfStock

B2M 适用: 人 FACS 宿主: 小鼠 Monoclonal B2M-01 FITC

Western Blotting Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01). (beta-2 Microglobulin 抗体 (FITC))

Flow cytometry analysis (surface staining) of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC. (beta-2 Microglobulin 抗体 (FITC))

FACS

Confocal microscopy of human β2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor® 647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control (A6-458-C100). (beta-2 Microglobulin 抗体 (FITC))

3 images

(4 references)

产品编号 ABIN93905

发货至: 中国

Datasheet as PDF

抗原 See all beta-2 Microglobulin (B2M) 抗体

beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
适用
210

51

30

27

9

4

4

3

3

2

2

1

1

1

1
人
宿主
126

112

9

6

2

1
小鼠
Compatible Secondaries
克隆类型
129

126

1
单克隆
标记
115

32

24

14

13

7

3

3

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This beta-2 Microglobulin antibody is conjugated to FITC
应用范围
130

104

78

67

59

40

40

30

26

18

17

14

13

10

6

4

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

原理

Anti-Hu beta2-Microglobulin FITC

特异性

The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M, extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

无交叉反应

小鸡, Cow, 犬, 小鼠, 兔

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Purified human beta2-microglobulin

克隆位点

B2M-01

亚型

IgG2a

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anti-beta-2-Microglobulin (B2M) (AA 22-119) antibody
B2M 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal A2 unconjugated

anti-beta-2-Microglobulin (B2M) (AA 22-119) antibody
B2M 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-beta-2-Microglobulin (B2M) antibody
B2M 适用: 人, Non-Human Primate FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rB2M-961 unconjugated Recombinant Antibody

anti-beta-2-Microglobulin (B2M) antibody
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anti-beta-2-Microglobulin (B2M) antibody
B2M 适用: 人, Non-Human Primate WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal B2M-961 unconjugated

anti-beta-2-Microglobulin (B2M) antibody
B2M 适用: 人 WB, FACS, ELISA, IP, IHC (p), ICC, RIA 宿主: 小鼠 Monoclonal B2M-01 unconjugated

anti-beta-2-Microglobulin (B2M) antibody (Biotin)
B2M 适用: 人 WB, FACS, ELISA, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal B2M-01 Biotin

anti-beta-2-Microglobulin (B2M) antibody
B2M 适用: 人, Non-Human Primate FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal B2M-961 unconjugated

应用备注

Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).

Khurana, Traum, Aivado, Wells, Guerrero, Grall, Libermann, Schachter: "Urine proteomic profiling of pediatric nephrotic syndrome." in: Pediatric nephrology (Berlin, Germany), Vol. 21, Issue 9, pp. 1257-65, (2006) (PubMed).

Lafon, Prehaud, Megret, Lafage, Mouillot, Roa, Moreau, Rouas-Freiss, Carosella: "Modulation of HLA-G expression in human neural cells after neurotropic viral infections." in: Journal of virology, Vol. 79, Issue 24, pp. 15226-37, (2005) (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).
抗原

beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

别名

beta2-Microglobulin (B2M 产品)

背景

Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M

基因ID

567

UniProt

P61769

途径

TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process