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beta-2 Microglobulin 抗体

Cited in 2+ publications. The 小鼠 单克隆 anti-beta-2 Microglobulin antibody (Clone B2M-02) (ABIN93900) specifically detects beta-2 Microglobulin in WB, FACS, ELISA, ICC 和 IHC (p). The antibody is reactive with 人 和 Pig samples.
产品编号 ABIN93900
发货至: 中国
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Quick Overview for beta-2 Microglobulin 抗体 (ABIN93900)

抗原

See all beta-2 Microglobulin (B2M) 抗体
beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

适用

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宿主

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小鼠

克隆类型

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单克隆

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This beta-2 Microglobulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

B2M-02
  • 原理

    Anti-Hu beta2-Microglobulin Purified

    交叉反应 (详细)

    Human, Porcine

    产品特性

    The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    Purified isolated human beta2-microglobulin.

    亚型

    IgG1
  • 应用备注

    Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval, recommended dilution: 5 μg/mL, positive tissue: liver.
    Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions preferred.
    Flow cytometry: Recommended dilution: 1 μg/mL, positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
    ELISA: Working dilution should be determinated by investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, (2001) (PubMed).

    Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).

  • 抗原

    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

    别名

    beta2-Microglobulin

    背景

    Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M

    基因ID

    567

    UniProt

    P61769

    途径

    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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