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alpha Fetoprotein 抗体

The 小鼠 单克隆 anti-alpha Fetoprotein antibody is suitable to detect alpha Fetoprotein in samples from 人. It has been validated for WB, ELISA, ICC, IHC (p) 和 RIA.
产品编号 ABIN93887
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for alpha Fetoprotein 抗体 (ABIN93887)

抗原

See all alpha Fetoprotein (AFP) 抗体
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This alpha Fetoprotein antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

克隆位点

AFP-11
  • 原理

    Anti-alpha-Fetoprotein Purified

    交叉反应 (详细)

    Human

    产品特性

    The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    purified alpha-Fetoprotein

    亚型

    IgG1
  • 应用备注

    ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: hepatocellular carcinoma, heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1 hour / RT, detection DAB.
    Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • 抗原

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    别名

    alpha-Fetoprotein

    背景

    Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP

    基因ID

    174

    UniProt

    P02771

    途径

    C21-Steroid Hormone Metabolic Process
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