PsbO 抗体
Cited in 2+ publications.
The 兔 多克隆 anti-PsbO antibody (ABIN7676202) specifically detects PsbO in IP, IHC 和 WB.
The antibody is reactive with Arabidopsis thaliana, Barley, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum 和 Zea mays samples.
1 image
(2 references)
产品编号 ABIN7676202
发货至:
中国
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Quick Overview for PsbO 抗体 (ABIN7676202)
抗原
PsbO
适用
Arabidopsis thaliana, Barley, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum, Zea mays
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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原理
- Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)
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交叉反应 （详细）
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Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942 -
预测反应
- Brassica oleracea,Pisum sativum,Populus tremula,Picea sitcHensis,Vitis vinifera
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纯化方法
- Serum
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免疫原
- N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321
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应用备注
- 1 : 1000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).
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: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
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- PsbO
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背景
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Background: The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
Additional information: Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody
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分子量
- 33 kDa
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基因ID
- 836789
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UniProt
- P23321, Q9S841
抗原
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