EXOS 抗体 (cleaved)
There is 1 publication for this product available. The 小鸡 多克隆 anti-EXOS antibody is suitable to detect EXOS in samples from Pseudomonas aeruginosa. It has been validated for WB.
(1 reference)
产品编号 ABIN93557
发货至:
中国
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Quick Overview for EXOS 抗体 (cleaved) (ABIN93557)
抗原
EXOS
(ExoS Gene Product (EXOS))
适用
Pseudomonas aeruginosa
宿主
小鸡
克隆类型
多克隆
应用范围
Western Blotting (WB)
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抗原表位
- cleaved
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原理
- Anti-ExoS | Exoenzyme S
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交叉反应 （详细）
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Pseudomonas aeruginosa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Pseudomonas aeruginosa
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纯化方法
- Purified, total IgY
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免疫原
- amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
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亚型
- IgY
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应用备注
- 1 : 5000 (WB)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C
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储存方法
- Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
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: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
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- EXOS (ExoS Gene Product (EXOS))
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别名
- Exoenzyme S (ExoS)
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背景
- Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
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分子量
- 48 kDa
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UniProt
- Q51449
抗原
-
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