NPT 抗体
Our Local Distributor
北京 101111
Quick Overview for NPT 抗体 (ABIN932234)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
免疫原
- NPT antibody was raised in Mouse using a purified recombinant fragment of NPT expressed in E. coli as the immunogen.
-
亚型
- IgG2a
-
-
-
-
应用备注
-
ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
-
注意事项
-
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
-
储存方法
- Store at 4 °C for short term storage. Store at -20 °C for long term storage.
-
-
- NPT
-
背景
- NPT is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPT is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents. Synonyms: Monoclonal NPT antibody, Anti-NPT antibody, Neomycin phosphotransferase II antibody, NptII antibody.
抗原
-