T7-Tag 抗体 (HRP)
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北京 101111
Quick Overview for T7-Tag 抗体 (HRP) (ABIN920811)
抗原
宿主
克隆类型
标记
应用范围
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序列
- MASMTGGQQM G
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应用备注
- Western blot: Use at 1:5,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 50 mM HEPES pH 7.0, 0.1 M sodium chloride with 0.2% BSA and 0.01% thimerosal.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- T7-Tag
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别名
- T7 tag
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物质类
- Tag
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背景
- Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support and then conjugated with horseradish peroxidase (HRP). Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. ELISA: The anti-T7 diluted 1:25,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier.
抗原
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