T7-Tag 抗体
Quick Overview for T7-Tag 抗体 (ABIN920809)
抗原
宿主
克隆类型
标记
应用范围
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序列
- MASMTGGQQM G
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纯化方法
- Affinity purified
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anti-T7-Tag antibody
WB 宿主: 小鼠 Monoclonal 1M4 unconjugated
anti-T7-Tag antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-T7-Tag antibodyWB 宿主: 小鼠 Monoclonal 1H5 unconjugated
anti-T7-Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot: Use at 1:5,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, pH 7.2 and 0.1% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- T7-Tag
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别名
- T7 tag
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物质类
- Tag
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背景
- Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.
抗原
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