DYKDDDDK Tag 抗体 (FITC)
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北京 101111
Quick Overview for DYKDDDDK Tag 抗体 (FITC) (ABIN920781)
抗原
适用
宿主
克隆类型
标记
应用范围
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序列
- DYKDDDDK
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应用备注
- ELISA: Use at 1:5,000 - 1:20,000. Immunofluorescence: Use at 1:250- 1:1,000. Western blot: Use at 1:1,000 - 1:5,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 1.0 mL of 1.0 mg/mL FITC-labeled antibody in PBS, pH 7.0 with protein stabilizer and 0.1% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- DYKDDDDK Tag
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别名
- Flag Tag
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物质类
- Tag
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背景
- Rabbits were immunized with the DYKDDDDK (DYKDDDDK) peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were affinity-purified using the peptide immobilized on a solid phase. The affinity-purified antibodies were then conjugated with Fluorescein Isothiocyanate (FITC). Prior to conjugation, the antibodies were analyzed by ELISA and Western blot.
抗原
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