E Tag 抗体
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北京 101111
Quick Overview for E Tag 抗体 (ABIN920773)
抗原
宿主
克隆类型
标记
应用范围
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序列
- GAPVPYPDPL EPR
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纯化方法
- Affinity purified
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anti-E Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 11H12B3 unconjugated
anti-E Tag antibody (HRP)WB, ELISA, ICC 宿主: 山羊 Polyclonal HRP
anti-E Tag antibody (FITC)ICC, IF 宿主: 山羊 Polyclonal FITC
anti-E Tag antibodyWB, ELISA, IP, ICC 宿主: 山羊 Polyclonal unconjugated
anti-E Tag antibodyWB, ELISA, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot: Recommend dilution range 1:1,000 - 1:3,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS pH 7.2 with 0.1% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- E Tag
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别名
- E-tag
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物质类
- Tag
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背景
- Rabbits were immunized with E-tag peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated donkey anti-rabbit (IgG) at 1:18,000 and TMB as the substrate. Appropriate specificity controls were run.
抗原
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