Flow cytometry: Use at a 1:100 - 1:500 dilution. ELISA: Use at a 1:100 - 1:1,000 dilution. Dot blot: Use at a 1:100 - 1:1,000 dilution. Western blot: Use at a 1:500 - 1:2,000 dilution. Immunohistochemistry: Use at a 1:100 - 1:500 dilution. Immunocytochemistry: Use at a 1:100 - 1:1,000 dilution. Immunoprecipitation: Use at a 1:100 - 1:500 dilution.