4-Hydroxy-2-Hexenal (4-HHE) 抗体
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Quick Overview for 4-Hydroxy-2-Hexenal (4-HHE) 抗体 (ABIN920668)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for HHE-modified protein (especially HHE-His Michael adduct)
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纯化方法
- Protein A purified.
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免疫原
- HHE-modified keyhole-lympet hemocyanine.
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亚型
- IgG1 kappa
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应用备注
- Immunohistochemistry: Recommended antibody concentration is 0.5 - 1.0 µg/mL on paraformaldehyde fixed tissue.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Monoclonal antibody at 100 µg/mL in 10 mM PBS containing 0.1 % NaN3 and protein stabilizer. Prepared as ascite.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- 4-Hydroxy-2-Hexenal (4-HHE)
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物质类
- Chemical
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背景
- 4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is a highly reactive aldehyde and reacts with histidine residue of protein to form Michael- addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enables detection of HHE-His in tissue samples.
抗原
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