Keratan Sulfate 抗体
This anti-Keratan Sulfate antibody is a 小鼠 单克隆 antibody detecting Keratan Sulfate in WB, IHC 和 ELISA. Suitable for 人.
Quick Overview for Keratan Sulfate 抗体 (ABIN870831)
抗原
See all Keratan Sulfate (KS) 抗体
Keratan Sulfate (KS)
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Keratan Sulfate antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
克隆位点
4B3-D10
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特异性
- Aggrecan is the major proteoglycan of human articular cartilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan sulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea. Monoclonal Ab 4B3/D10 is specific for keratan sulfate glycosaminoglycan chains. Preliminary results based on Western Blot analysis of keratan sulfate from different sources indicate that mAb 4B3/10 is specific for keratan sulfate from articular cartilage and shows only minimal if any crossreactivity with keratan sulfate from intervertebral disc. This is in contrast to the epitope recognized by the known mAb 5D4, which recognizes a widely distributed keratan sulfate epitope. Therefore, in addition to immunohistochemistry, the mAb 4B3 / D10 is well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
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纯化方法
- Purified human aggrecan. Protein G purified.
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亚型
- IgG1 kappa
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应用备注
- ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml) This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Resuspend in aqua bidest.
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缓冲液
- 50 mM TRIS pH 7.4
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储存条件
- 4 °C
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- Keratan Sulfate (KS)
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别名
- Keratan-Sulfate
抗原
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