Myosin IB 抗体 (Internal Region)
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Quick Overview for Myosin IB 抗体 (Internal Region) (ABIN870635)
抗原
See all Myosin IB (MYO1B) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- MYO1B
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序列
- RTVEAKQEKV STT
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特异性
- This antibody is expected to recognize both reported isoforms (NP_001123630.1, NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RTVEAKQEKVSTT, from the internal region of the protein sequence according to NP_001123630.1, NP_036355.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10 μg/mL.
Western Blot: Approx 120 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125 kDa according to Human NP_036355.2 and 128 kDa according to Mouse NP_034993.2). Recommended concentration: 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Myosin IB (MYO1B)
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别名
- MYO1B
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背景
- MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-I alpha, myosin-Ib
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分子量
- 125kDa
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基因ID
- 4430, 17912, 117057
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NCBI登录号
- NP_001123630, NP_036355
抗原
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