IFNAR2 抗体 (Carboxyfluorescein (CFS))
Quick Overview for IFNAR2 抗体 (Carboxyfluorescein (CFS)) (ABIN796985)
抗原
See all IFNAR2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse IFNA/B R2.
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纯化方法
- Immunoaffinity purified
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免疫原
- Mouse myeloma cell line NS0- derived recombinant human IFN-a/b R2 extracellular domain
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亚型
- IgG
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anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 165-260) antibody
IFNAR2 适用: 人, 大鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 27-243) antibodyIFNAR2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 27-243) antibodyIFNAR2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (Internal Region) antibodyVerified IFNAR2 适用: 人 ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) antibodyIFNAR2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (Center) antibodyIFNAR2 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 27-243) antibodyIFNAR2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (C-Term) antibodyIFNAR2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (N-Term) antibodyIFNAR2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 27-243) antibodyIFNAR2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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实验流程
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Flow Cytometry Validation
This antibody has been tested for flow cytometry using A20 cells.- Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
- After blocking, 10ul of conjugated antibody was added to 1 - 2.5 x 10e5 cells and incubated for 30 minutes at room temperature.
- Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
- The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
- Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.
- Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, up to 0.5 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles and prolonged exposure to light.
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储存条件
- 4 °C
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储存方法
- Store at 4°C for up to 1 year.
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有效期
- 12 months
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- IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))
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别名
- IFNAR2
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背景
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Name/Gene ID: IFNAR2
Family: Interferon Receptor
Synonyms: IFNAR2, IFN-alpha/beta receptor 2, IFN-alpha-REC, IFN-alpha binding protein, IFN-R, IFN-R-2, IFNARB, Type I interferon receptor 2, IFNABR -
基因ID
- 3455
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UniProt
- P48551
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途径
- JAK/STAT Signaling, Hepatitis C
抗原
-
